A Radioreceptor Assay for Catecholamines
نویسندگان
چکیده
منابع مشابه
A Radioreceptor Assay for Quantitating Plasma
A sensitive and precise radioreceptor assay for determining plasma levels of human factor Vill/von Willebrand’s factor (FVIII/vWF) has been developed by taking advantage of the FVliI/vWF receptor sites on human platelets. Paraformaldehyde-fixed platelets. which were processed and then stored. retained FVIII/vWF binding activity and therefore could be used as a convenient source of receptors. Th...
متن کاملInsulin radioreceptor assay for human erythrocytes.
Human erythrocytes have specific insulin receptors. A radloreceptor assay for the determination of Insulin binding to these receptors is presented. After two passages over a Boyum-type adient, erythrocytes from freshly collected heparinized blood were isolated and 3.5 X iO erythrocytes per milliliterwere incubated for 2.5 h in a modified pH 8.0 4-(2-hydroxyethyl)1-piperazine ethane sulfonate bu...
متن کاملA Radioreceptor Assay for Quantitating Plasma Factor Vill
A sensitive and precise radioreceptor assay for determining plasma levels of human factor Vill/von Willebrand’s factor (FVIII/vWF) has been developed by taking advantage of the FVliI/vWF receptor sites on human platelets. Paraformaldehyde-fixed platelets. which were processed and then stored. retained FVIII/vWF binding activity and therefore could be used as a convenient source of receptors. Th...
متن کاملSpecificities compared for a radioreceptor assay and a radioimmunoassay of atrial natriuretic peptide.
The main active form of atrial natriuretic peptide (ANP) in the circulation is alpha ANP(1-28) [gamma-ANP(99-126)], although some active and inactive metabolites are also though to be present. Some immunoassays for alpha ANP reportedly detect inactive metabolites, as determined by their comparison with results by receptor assay, which would lead to misleading results for alpha ANP(1-28) measure...
متن کاملRefinement of a radioreceptor binding assay for nicotinic acid adenine dinucleotide phosphate
The measurement of changes in nicotinic acid adenine dinucleotide phosphate (NAADP) levels in cells has been, and remains, key to the investigation of the functions of NAADP as a Ca2+ -releasing second messenger. Here we provide details of how to isolate NAADP from cells by extraction with perchloric acid and then measure the NAADP using a radioreceptor assay. We demonstrate that NAADP is neith...
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ژورنال
عنوان ژورنال: Folia Endocrinologica Japonica
سال: 1980
ISSN: 0029-0661
DOI: 10.1507/endocrine1927.56.12_1655